In the search for beneficial traits that may allow crops to resist abiotic and biotic stresses, fast and accurate methods are required for efficient and effective plant high-throughput phenotyping. Such methods must involve automated measurements of plant morphology, biochemistry and physiology to determine potential and actual yield under a variety of monitored environmental conditions.
With over 20 years’ experience of designing instrumentation for plant imaging PSI is now at the forefront of providing complete solutions PlantScreenTM Phenotyping Systems for automated multidimensional plant phenotyping.
PlantScreenTM Phenotyping Systems designed for integrative phenotyping on temporal and spatial level are proven in a range of applications across the world:
PlantScreenTM phenotyping systems can be used to both induce defined stresses and identify the degree to which plants are resistant or susceptible. Analysis of chlorophyll fluorescence kinetics is key to identifying the precise point at which plants begin to respond to stress conditions and the degree to which these conditions inhibit photochemical efficiency. RGB and morphometric analyses quantify effects on growth, whereas NIR and thermal imaging indicate water status and stomatal closure.
Infection of crop plants by pathogens is a major factor contributing to reduction in crop yields. The PlantScreenTM systems allow accurate quantification of the degree to which crop varieties are susceptible to pathogen attack, providing an extremely valuable tool in breeding programs and screening of GMOs. For example, RGB analysis quantifies the time course of plant fungal infection, while measurement of chlorophyll fluorescence kinetics shows how the infection limits photochemical efficiency and potential yield. Repeated morphometric analysis quantifies reduced growth and effects on plant architecture.
PlantScreenTM systems incorporate high resolution cameras to monitor numerous aspects of plant growth and morphology throughout the development of plants. Each plant, whether it is in a pot and measured separately, or in a tray and measured together with other samples, is tracked individually so that the time course of changes in growth parameters and plant architecture, can analyzed with respect to developmental stage. Both monocots and dicots may be screened. PlantScreenTM systems are available in several sizes and configurations to track the growth of very small (e.g. Arabidopsis) and very large (e.g. mature corn) samples.
Nutrient Stress and Ecotoxicology
The PlantScreenTM system has the option for delivery of specific nutrient regimes to plants so that effects of nutrient deficiency can be studied throughout the growth cycle. In addition, the nutrient delivery system can be used to provide doses of heavy metals, or other potentially toxic substances, so that tolerance or susceptibility to such stresses may be screened.
The systems may be used with aquatic plants, such as duckweeds (Lemnodeae), for quantitative testing of ecotoxilogical challenges, as may be required by international regulatory bodies. Relevant measurements include chlorophyll kinetic imaging to determine how nutrient or chemical challenges affect the photosynthetic physiology and efficiency of the plants. RGB and morphometric images analysis enables the monitoring of plant color and growth parameters. All data are immediately available for detailed analysis within the PlantScreenTM software, or for numerical download to other programs for mathematical and statistical analysis.